Non-contacted bases affect the affinity of synthetic P22 operators for P22 repressor.
نویسندگان
چکیده
منابع مشابه
Structural evolution of the P22-like phages: comparison of Sf6 and P22 procapsid and virion architectures.
Coat proteins of tailed, dsDNA phages and in herpesviruses include a conserved core similar to the bacteriophage HK97 subunit. This core is often embellished with other domains such as the telokin Ig-like domain of phage P22. Eighty-six P22-like phages and prophages with sequenced genomes share a similar set of virion assembly genes and, based on comparisons of twelve viral assembly proteins (s...
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Phage P22 is known to have a linear duplex chromosome that is circularly permuted and terminally repeated. We have found, by constructing a partial denaturation map of mature P22 DNA, that circular permutation in P22 DNA is restricted: all of the ends of the mature DNA fall within 20% of each other on the physical map. The limited distribution of ends can be explained by the “headful” packaging...
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We report the first accurate genome sequence for bacteriophage P22, correcting a 0.14% error rate in previously determined sequences. DNA sequencing technology is now good enough that genomes of important model systems like P22 can be sequenced with essentially 100% accuracy with minimal investment of time and resources.
متن کاملP22 c2 repressor-operator complex: mechanisms of direct and indirect readout.
The P22 c2 repressor protein (P22R) binds to DNA sequence-specifically and helps to direct the temperate lambdoid bacteriophage P22 to the lysogenic developmental pathway. We describe the 1.6 A X-ray structure of the N-terminal domain (NTD) of P22R in a complex with a DNA fragment containing the synthetic operator sequence [d(ATTTAAGATATCTTAAAT)]2. This operator has an A-T base pair at position...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1992
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)50399-4